A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus).
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ABSTRACT:
SUBMITTER: Sun B
PROVIDER: S-EPMC1899364 | biostudies-literature | 2007 Mar
REPOSITORIES: biostudies-literature
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A single residue change in Vibrio harveyi hemolysin results in the loss of phospholipase and hemolytic activities and pathogenicity for turbot (Scophthalmus maximus).
Sun Boguang B Zhang Xiao-Hua XH Tang Xuexi X Wang Shushan S Zhong Yingbin Y Chen Jixiang J Austin Brian B
Journal of bacteriology 20070112 6
Vibrio harveyi hemolysin, an important virulence determinant in fish pathogenesis, was further characterized, and the enzyme was identified as a phospholipase B by gas chromatography. Site-directed mutagenesis revealed that a specific residue, Ser153, was critical for its enzymatic activity and for its virulence in fish. ...[more]
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