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Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi hemolysin VHH.

ABSTRACT: Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, and the signal peptide.

SUBMITTER: Zhong Y 

PROVIDER: S-EPMC1594901 | biostudies-literature | 2006 Oct

REPOSITORIES: biostudies-literature

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Overexpression, purification, characterization, and pathogenicity of Vibrio harveyi hemolysin VHH.

Zhong Yingbin Y   Zhang Xiao-Hua XH   Chen Jixiang J   Chi Zhenghao Z   Sun Boguang B   Li Yun Y   Austin Brian B  

Infection and immunity 20061001 10

Vibrio harveyi VHH hemolysin is a putative pathogenicity factor in fish. In this study, the hemolysin gene vhhA was overexpressed in Escherichia coli, and the purified VHH was characterized with regard to pH and temperature profiles, phospholipase activity, cytotoxicity, pathogenicity to flounder, and the signal peptide. ...[more]

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