Ontology highlight
ABSTRACT:
SUBMITTER: Seipp MT
PROVIDER: S-EPMC1899416 | biostudies-literature | 2007 Jul
REPOSITORIES: biostudies-literature
Seipp Michael T MT Durtschi Jacob D JD Liew Michael A MA Williams Jamie J Damjanovich Kristy K Pont-Kingdon Genevieve G Lyon Elaine E Voelkerding Karl V KV Wittwer Carl T CT
The Journal of molecular diagnostics : JMD 20070701 3
Amplicon melting is a closed-tube method for genotyping that does not require probes, real-time analysis, or allele-specific polymerase chain reaction. However, correct differentiation of homozygous mutant and wild-type samples by melting temperature (Tm) requires high-resolution melting and closely controlled reaction conditions. When three different DNA extraction methods were used to isolate DNA from whole blood, amplicon Tm differences of 0.03 to 0.39 degrees C attributable to the extraction ...[more]