Ontology highlight
ABSTRACT:
SUBMITTER: Dames S
PROVIDER: S-EPMC1899421 | biostudies-literature | 2007 Jul
REPOSITORIES: biostudies-literature
Dames Shale S Margraf Rebecca L RL Pattison David C DC Wittwer Carl T CT Voelkerding Karl V KV
The Journal of molecular diagnostics : JMD 20070701 3
An unlabeled probe assay relies on a double-stranded DNA-binding dye to detect and verify target based on amplicon and probe melting. During the development and application of unlabeled probe assays, aberrant melting peaks are sometimes observed that may interfere with assay interpretation. In this report, we investigated the origin of aberrant melting profiles observed in an unlabeled probe assay for exon 10 of the RET gene. It was determined that incomplete 3' blocking of the unlabeled probe a ...[more]