Ontology highlight
ABSTRACT:
SUBMITTER: Prieto J
PROVIDER: S-EPMC1904291 | biostudies-literature | 2007
REPOSITORIES: biostudies-literature
Prieto Jesús J Redondo Pilar P Padró Daniel D Arnould Sylvain S Epinat Jean-Charles JC Pâques Frédéric F Blanco Francisco J FJ Montoya Guillermo G
Nucleic acids research 20070422 10
Meganucleases are sequence-specific endonucleases with large cleavage sites that can be used to induce efficient homologous gene targeting in cultured cells and plants. These enzymes open novel perspectives for genome engineering in a wide range of fields, including gene therapy. A new crystal structure of the I-CreI dimer without DNA has allowed the comparison with the DNA-bound protein. The C-terminal loop displays a different conformation, which suggests its implication in DNA binding. A site ...[more]