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Protection of mice against rotavirus challenge following intradermal DNA immunization by Biojector needle-free injection.


ABSTRACT: Mucosal administration (intranasal or oral) of a VP6 rotavirus vaccine to mice consistently elicits high levels of protection after rotavirus challenge (93->99% reductions in fecal rotavirus shedding) but only when co-administered with an effective adjuvant such as LT(R192G). Here, we showed that Biojector needle-free injection of VP6-encoded plasmids also induced protection (85-93%) when they were co-administrated with LT(R192G)-encoded plasmids. A reduction in the amount of VP6 plasmid from 50 to 10 microg reduced protection from 93 to 70%, but the immunized mice remained significantly (P<0.05) protected. Intramuscular needle injection of VP6/LT(R192G)-plasmids also induced significant protection (66%).

SUBMITTER: Choi AH 

PROVIDER: S-EPMC1906844 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature

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Protection of mice against rotavirus challenge following intradermal DNA immunization by Biojector needle-free injection.

Choi Anthony H-C AH   Smiley Kristi K   Basu Mitali M   McNeal Monica M MM   Shao Mingyuan M   Bean Judy A JA   Clements John D JD   Stout Richard R RR   Ward Richard L RL  

Vaccine 20070122 16


Mucosal administration (intranasal or oral) of a VP6 rotavirus vaccine to mice consistently elicits high levels of protection after rotavirus challenge (93->99% reductions in fecal rotavirus shedding) but only when co-administered with an effective adjuvant such as LT(R192G). Here, we showed that Biojector needle-free injection of VP6-encoded plasmids also induced protection (85-93%) when they were co-administrated with LT(R192G)-encoded plasmids. A reduction in the amount of VP6 plasmid from 50  ...[more]

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