Ontology highlight
ABSTRACT:
SUBMITTER: D'Alfonso L
PROVIDER: S-EPMC1914445 | biostudies-literature | 2007 Jul
REPOSITORIES: biostudies-literature
D'Alfonso Laura L Collini Maddalena M Cannone Fabio F Chirico Giuseppe G Campanini Barbara B Cottone Grazia G Cordone Lorenzo L
Biophysical journal 20070406 1
We report investigations on the properties of nanoenvironments around single-GFP-mut2 proteins in trehalose-water matrixes. Single-GFPmut2 molecules embedded in thin trehalose-water films were characterized in terms of their fluorescence brightness, bleaching dynamics, excited state lifetime, and fluorescence polarization. For each property, sets of approximately 100-150 single molecules have been investigated as a function of trehalose content and hydration. Three distinct and interconverting f ...[more]