Ontology highlight
ABSTRACT:
SUBMITTER: Lam PP
PROVIDER: S-EPMC1924827 | biostudies-literature | 2007 Jul
REPOSITORIES: biostudies-literature
Lam Patrick P L PP Hyvärinen Kati K Kauppi Maria M Cosen-Binker Laura L Laitinen Saara S Keränen Sirkka S Gaisano Herbert Y HY Olkkonen Vesa M VM
Molecular biology of the cell 20070418 7
We identified in a yeast two-hybrid screen the EF-hand Ca(2+)-binding protein Cab45 as an interaction partner of Munc18b. Although the full-length Cab45 resides in Golgi lumen, we characterize a cytosolic splice variant, Cab45b, expressed in pancreatic acini. Cab45b is shown to bind (45)Ca(2+), and, of its three EF-hand motifs, EF-hand 2 is demonstrated to be crucial for the ion binding. Cab45b is shown to interact with Munc18b in an in vitro assay, and this interaction is enhanced in the presen ...[more]