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MiR-200b mediates post-transcriptional repression of ZFHX1B.

ABSTRACT: MicroRNAs have important functions during animal development and homeostasis through post-transcriptional regulation of their cognate mRNA targets. ZFHX1B is a transcriptional repressor involved in the TGFbeta signaling pathway and in processes of epithelial to mesenchymal transition via regulation of E-cadherin. We show that Zfhx1b and miR-200b are regionally coexpressed in the adult mouse brain and that miR-200b represses the expression of Zfhx1b via multiple sequence elements present in the 3'-untranslated region. Overexpression of miR-200b leads to repression of endogenous ZFHX1B, and inhibition of miR-200b relieves the repression of ZFHX1B. In accordance with these findings, miR-200b regulates the activity of the E-cadherin promoter.

SUBMITTER: Christoffersen NR

PROVIDER: S-EPMC1924904 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature

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miR-200b mediates post-transcriptional repression of ZFHX1B.

Christoffersen Nanna Rønbjerg NR Silahtaroglu Asli A Orom Ulf Andersson UA Kauppinen Sakari S Lund Anders H AH

RNA (New York, N.Y.) 20070621 8

MicroRNAs have important functions during animal development and homeostasis through post-transcriptional regulation of their cognate mRNA targets. ZFHX1B is a transcriptional repressor involved in the TGFbeta signaling pathway and in processes of epithelial to mesenchymal transition via regulation of E-cadherin. We show that Zfhx1b and miR-200b are regionally coexpressed in the adult mouse brain and that miR-200b represses the expression of Zfhx1b via multiple sequence elements present in the 3 ...[more]

PMID: 17585049

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