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A PCR-based method for detection and quantification of small RNAs.

ABSTRACT: Recent cloning efforts have identified hundreds of thousands of small RNAs including micro RNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs). These non-coding small RNAs need to be further validated and characterized by detecting and quantifying their expression in different tissues and during different developmental courses. A simple, accurate, and sensitive method for small RNA expression profiling is in high demand. Here, we report such a PCR-based method.

SUBMITTER: Ro S

PROVIDER: S-EPMC1934510 | biostudies-literature | 2006 Dec

REPOSITORIES: biostudies-literature

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A PCR-based method for detection and quantification of small RNAs.

Ro Seungil S Park Chanjae C Jin Jingling J Sanders Kenton M KM Yan Wei W

Biochemical and biophysical research communications 20061030 3

Recent cloning efforts have identified hundreds of thousands of small RNAs including micro RNAs (miRNAs), Piwi-interacting RNAs (piRNAs), and small nucleolar RNAs (snoRNAs). These non-coding small RNAs need to be further validated and characterized by detecting and quantifying their expression in different tissues and during different developmental courses. A simple, accurate, and sensitive method for small RNA expression profiling is in high demand. Here, we report such a PCR-based method. ...[more]

PMID: 17084816

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