Ontology highlight
ABSTRACT:
SUBMITTER: Sharma SK
PROVIDER: S-EPMC1937568 | biostudies-literature | 2007 Jul
REPOSITORIES: biostudies-literature
Sharma Shail K SK Nirenberg Marshall M
Proceedings of the National Academy of Sciences of the United States of America 20070723 31
Cultures of neuroblasts that generate abundant neurons were established from Drosophila embryos to study silencing of genes by RNA interference (RNAi). Cultured cells expressed ELAV, a marker of neurons, Futsch, a marker of neurites, and Synapsin, Synaptobrevin, and Synaptogamin, proteins involved in neurotransmitter secretion. Conditions were found for efficient transfection of cells with siRNAs for ELAV or the insulin-like receptor, which resulted in marked decreases in neurons that express EL ...[more]