Plasmodium falciparum: a novel method for analyzing haplotypes in mixed infections.
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ABSTRACT:
SUBMITTER: Certain LK
PROVIDER: S-EPMC1950286 | biostudies-literature | 2007 Mar
REPOSITORIES: biostudies-literature
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Plasmodium falciparum: a novel method for analyzing haplotypes in mixed infections.
Certain Laura K LK Sibley Carol H CH
Experimental parasitology 20061017 3
Studying the population genetics of Plasmodium falciparum is necessary for understanding the spread of drug resistance. However, these studies are hampered by the inability to determine haplotypes from patient samples that contain multiple parasite populations. Therefore, we have developed a method for separating for genetic analysis the individual strains in a mixed infection. We amplified a 6 kb region of chromosome 4, including the dihydrofolate reductase gene and upstream microsatellite mark ...[more]
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