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Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach.

ABSTRACT: To enhance the sensitivity of the available real-time PCR systems for the detection of Mycoplasma pneumoniae, we established a method to amplify copies of the repetitive element repMp1. In a study of respiratory tract samples, we found that, compared to the use of the conserved part of the P1 adhesin gene as a monocopy target, the use of the repMp1-PCR showed an increase in the detected genome equivalents by a factor of 22.

SUBMITTER: Dumke R

PROVIDER: S-EPMC1951254 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature

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Sensitive detection of Mycoplasma pneumoniae in human respiratory tract samples by optimized real-time PCR approach.

Dumke Roger R Schurwanz Nicol N Lenz Matthias M Schuppler Markus M Lück Christian C Jacobs Enno E

Journal of clinical microbiology 20070530 8

To enhance the sensitivity of the available real-time PCR systems for the detection of Mycoplasma pneumoniae, we established a method to amplify copies of the repetitive element repMp1. In a study of respiratory tract samples, we found that, compared to the use of the conserved part of the P1 adhesin gene as a monocopy target, the use of the repMp1-PCR showed an increase in the detected genome equivalents by a factor of 22. ...[more]

PMID: 17537933

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