Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation.
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ABSTRACT:
SUBMITTER: Winchell JM
PROVIDER: S-EPMC2546712 | biostudies-literature | 2008 Sep
REPOSITORIES: biostudies-literature
Publications
Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation.
Winchell Jonas M JM Thurman Kathleen A KA Mitchell Stephanie L SL Thacker W Lanier WL Fields Barry S BS
Journal of clinical microbiology 20080709 9
We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions of Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase testing confidence. ...[more]
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