Determinants of entry exclusion within Eex and TraG are cytoplasmic.
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ABSTRACT:
SUBMITTER: Marrero J
PROVIDER: S-EPMC1951900 | biostudies-literature | 2007 Sep
REPOSITORIES: biostudies-literature
Publications
Determinants of entry exclusion within Eex and TraG are cytoplasmic.
Marrero Joeli J Waldor Matthew K MK
Journal of bacteriology 20070615 17
We report here functional and topological analyses of TraG and Eex, the donor and recipient cell inner membrane proteins that mediate entry exclusion in the SXT/R391 family of integrative conjugative elements. We found that the exclusion-determining regions of the Eex variants EexS (SXT) and EexR (R391) are located in distinct yet overlapping regions of the proteins. Unexpectedly, the carboxyl-terminal regions of TraG and Eex, which contain the residues essential for exclusion activity and speci ...[more]
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