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Caenorhabditis elegans SID-2 is required for environmental RNA interference.

ABSTRACT: In plants and in the nematode Caenorhabditis elegans, an RNAi signal can trigger gene silencing in cells distant from the site where silencing is initiated. In plants, this signal is known to be a form of dsRNA, and the signal is most likely a form of dsRNA in C. elegans as well. Furthermore, in C. elegans, dsRNA present in the environment or expressed in ingested bacteria is sufficient to trigger RNAi (environmental RNAi). Ingestion and soaking delivery of dsRNA has also been described for other invertebrates. Here we report the identification and characterization of SID-2, an intestinal luminal transmembrane protein required for environmental RNAi in C. elegans. SID-2, when expressed in the environmental RNAi defective species Caenorhabditis briggsae, confers environmental RNAi.

SUBMITTER: Winston WM

PROVIDER: S-EPMC1965553 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature

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Caenorhabditis elegans SID-2 is required for environmental RNA interference.

Winston William M WM Sutherlin Marie M Wright Amanda J AJ Feinberg Evan H EH Hunter Craig P CP

Proceedings of the National Academy of Sciences of the United States of America 20070611 25

In plants and in the nematode Caenorhabditis elegans, an RNAi signal can trigger gene silencing in cells distant from the site where silencing is initiated. In plants, this signal is known to be a form of dsRNA, and the signal is most likely a form of dsRNA in C. elegans as well. Furthermore, in C. elegans, dsRNA present in the environment or expressed in ingested bacteria is sufficient to trigger RNAi (environmental RNAi). Ingestion and soaking delivery of dsRNA has also been described for othe ...[more]

PMID: 17563372

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