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Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans.

ABSTRACT: CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 A, and diffract to 2.2 A resolution using synchrotron radiation.

SUBMITTER: Tricot C

PROVIDER: S-EPMC1978110 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature

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Overexpression, purification, crystallization and crystallographic analysis of CopK of Cupriavidus metallidurans.

Tricot Catherine C van Aelst Sebastien S Wattiez Ruddy R Mergeay Max M Stalon Victor V Wouters Johan J

Acta crystallographica. Section F, Structural biology and crystallization communications 20050831 Pt 9

CopK of Cupriavidus metallidurans is a 93-amino-acid protein whose mature form (73 amino acids) has been purified and crystallized by the hanging-drop vapour-diffusion method in 100 mM citrate pH 3.5, 200 mM Li2SO4, 20%(w/v) glycerol, 13%(w/v) PEG 8000. Crystals display orthorhombic symmetry, with unit-cell parameters a = 57.53, b = 128.65, c = 49.77 A, and diffract to 2.2 A resolution using synchrotron radiation. ...[more]

PMID: 16511169

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