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Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp6.

ABSTRACT: Enpp (ectonucleotide phosphodiesterase/pyrophosphatase) 6 is a membrane-bound glycoprotein that hydrolyzes choline-containing compounds such as lysophosphatidylcholine and glycerophosphorylcholine, and presumably participates in choline metabolism. The catalytic domain of mouse Enpp6 was expressed in HEK293T cells, purified using the TARGET tag/P20.1-Sepharose system and crystallized. An X-ray diffraction data set was collected to 1.8?Å resolution. The crystal belonged to space group P1, with unit-cell parameters a=63.7, b=68.8, c=69.7?Å, ?=60.6, ?=87.0, ?=68.1°. Assuming the presence of two protein molecules per asymmetric unit, the solvent content was estimated to be 49.5%.

SUBMITTER: Morita J

PROVIDER: S-EPMC4051540 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Enpp6.

Morita Junko J Kato Kazuki K Mihara Emiko E Ishitani Ryuichiro R Takagi Junichi J Nishimasu Hiroshi H Aoki Junken J Nureki Osamu O

Acta crystallographica. Section F, Structural biology communications 20140524 Pt 6

Enpp (ectonucleotide phosphodiesterase/pyrophosphatase) 6 is a membrane-bound glycoprotein that hydrolyzes choline-containing compounds such as lysophosphatidylcholine and glycerophosphorylcholine, and presumably participates in choline metabolism. The catalytic domain of mouse Enpp6 was expressed in HEK293T cells, purified using the TARGET tag/P20.1-Sepharose system and crystallized. An X-ray diffraction data set was collected to 1.8 Å resolution. The crystal belonged to space group P1, with un ...[more]

PMID: 24915096

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