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Purification, crystallization and preliminary X-ray analysis of a hexameric beta-glucosidase from wheat.

ABSTRACT: The wheat beta-glucosidase TaGlu1b, which is only active in a hexameric form, was tagged with 6xHis at the N-terminus, overexpressed in Escherichia coli and purified in two steps. The protein complexed with a substrate aglycone was crystallized at 293 K from a solution containing 10 mM HEPES pH 7.2, 1 M LiSO4 and 150 mM NaCl using the hanging-drop vapour-diffusion method. Diffraction data were collected to 1.7 A at the Photon Factory. The crystal belongs to space group P4(1)32, with unit-cell parameters a = b = c = 194.65 A, alpha = beta = gamma = 90 degrees. The asymmetric unit was confirmed by molecular-replacement solution to contain one monomer, giving a solvent content of 72.1%.

SUBMITTER: Sue M

PROVIDER: S-EPMC1978116 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature

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Purification, crystallization and preliminary X-ray analysis of a hexameric beta-glucosidase from wheat.

Sue Masayuki M Yamazaki Kana K Kouyama Jun-ichi J Sasaki Yasuyuki Y Ohsawa Kanju K Miyamoto Toru T Iwamura Hajime H Yajima Shunsuke S

Acta crystallographica. Section F, Structural biology and crystallization communications 20050831 Pt 9

The wheat beta-glucosidase TaGlu1b, which is only active in a hexameric form, was tagged with 6xHis at the N-terminus, overexpressed in Escherichia coli and purified in two steps. The protein complexed with a substrate aglycone was crystallized at 293 K from a solution containing 10 mM HEPES pH 7.2, 1 M LiSO4 and 150 mM NaCl using the hanging-drop vapour-diffusion method. Diffraction data were collected to 1.7 A at the Photon Factory. The crystal belongs to space group P4(1)32, with unit-cell pa ...[more]

PMID: 16511181

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