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Expression, purification, crystallization and preliminary X-ray crystallographic analysis of tomato ?-galactosidase 4.

ABSTRACT: Plant ?-galactosidases play important roles in carbohydrate-reserve mobilization, cell-wall expansion and degradation, and turnover of signalling molecules during ripening. Tomato ?-galactosidase 4 (TBG4) not only has ?-galactosidase activity but also has exo-?-(1,4)-galactanase activity, and prefers ?-(1,4)-galactans longer than pentamers as its substrates; most other ?-galactosidases only have the former activity. Recombinant TBG4 protein expressed in the yeast Pichia pastoris was crystallized by the sitting-drop vapour-diffusion method using PEG 10,000 as a precipitant. The crystals belonged to the orthorhombic space group P212121, with unit-parameters a = 92.82, b = 96.30, c = 159.26 Å, and diffracted to 1.65 Å resolution. Calculation of the Matthews coefficient suggested the presence of two monomers per asymmetric unit (VM = 2.2 Å(3) Da(-1)), with a solvent content of 45%.

SUBMITTER: Eda M

PROVIDER: S-EPMC4321468 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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Expression, purification, crystallization and preliminary X-ray crystallographic analysis of tomato β-galactosidase 4.

Eda Masahiro M Ishimaru Megumi M Tada Toshiji T

Acta crystallographica. Section F, Structural biology communications 20150128 Pt 2

Plant β-galactosidases play important roles in carbohydrate-reserve mobilization, cell-wall expansion and degradation, and turnover of signalling molecules during ripening. Tomato β-galactosidase 4 (TBG4) not only has β-galactosidase activity but also has exo-β-(1,4)-galactanase activity, and prefers β-(1,4)-galactans longer than pentamers as its substrates; most other β-galactosidases only have the former activity. Recombinant TBG4 protein expressed in the yeast Pichia pastoris was crystallized ...[more]

PMID: 25664788

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