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Crystallization and preliminary crystallographic analysis of the (cytosine-5)-DNA methyltransferase NlaX from Neisseria lactamica.

ABSTRACT: Crystals of the (cytosine-5)-DNA methyltransferase NlaX from Neisseria lactamica (molecular weight 36.5 kDa) have been grown at 291 K using 2.5 M NaCl as precipitant. The crystals diffract to 3.0 A resolution at 100 K. The crystals belong to space group P321, with unit-cell parameters a = 121.98, b = 121.98, c = 56.71 A. There is one molecule in the asymmetric unit and the solvent content is estimated to be 62.1% by volume.

SUBMITTER: Kachalova GS

PROVIDER: S-EPMC1978117 | biostudies-literature | 2005 Sep

REPOSITORIES: biostudies-literature

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Crystallization and preliminary crystallographic analysis of the (cytosine-5)-DNA methyltransferase NlaX from Neisseria lactamica.

Kachalova Galina S GS Artyukh Rimma I RI Lavrova Natalia V NV Ryazanova Elena M EM Karyagina Anna S AS Kubareva Elena A EA Bartunik Hans D HD

Acta crystallographica. Section F, Structural biology and crystallization communications 20050831 Pt 9

Crystals of the (cytosine-5)-DNA methyltransferase NlaX from Neisseria lactamica (molecular weight 36.5 kDa) have been grown at 291 K using 2.5 M NaCl as precipitant. The crystals diffract to 3.0 A resolution at 100 K. The crystals belong to space group P321, with unit-cell parameters a = 121.98, b = 121.98, c = 56.71 A. There is one molecule in the asymmetric unit and the solvent content is estimated to be 62.1% by volume. ...[more]

PMID: 16511177

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