Ontology highlight
ABSTRACT:
SUBMITTER: El-Shami M
PROVIDER: S-EPMC2000319 | biostudies-literature | 2007 Oct
REPOSITORIES: biostudies-literature
El-Shami Mahmoud M Pontier Dominique D Lahmy Sylvie S Braun Laurence L Picart Claire C Vega Danielle D Hakimi Mohamed-Ali MA Jacobsen Steven E SE Cooke Richard R Lagrange Thierry T
Genes & development 20071001 20
Two forms of RNA Polymerase IV (PolIVa/PolIVb) have been implicated in RNA-directed DNA methylation (RdDM) in Arabidopsis. Prevailing models imply a distinct function for PolIVb by association of Argonaute4 (AGO4) with the C-terminal domain (CTD) of its largest subunit NRPD1b. Here we show that the extended CTD of NRPD1b-type proteins exhibits conserved Argonaute-binding capacity through a WG/GW-rich region that functionally distinguishes Pol IVb from Pol IVa, and that is essential for RdDM. Sit ...[more]