Ontology highlight
ABSTRACT:
SUBMITTER: Zwart G
PROVIDER: S-EPMC201233 | biostudies-literature | 2003 Oct
REPOSITORIES: biostudies-literature
Zwart Gabriel G van Hannen Erik J EJ Kamst-van Agterveld Miranda P MP Van der Gucht Katleen K Lindström Eva S ES Van Wichelen Jeroen J Lauridsen Torben T Crump Byron C BC Han Suk-Kyun SK Declerck Steven S
Applied and environmental microbiology 20031001 10
The identification of phylogenetic clusters of bacteria that are common in freshwater has provided a basis for probe design to target important freshwater groups. We present a set of 16S ribosomal RNA gene-based oligonucleotide probes specific for 15 of these freshwater clusters. The probes were applied in reverse line blot hybridization, a simple method that enables the rapid screening of PCR products from many samples against an array of probes. The optimized assay was made stringent to discri ...[more]