Ontology highlight
ABSTRACT:
SUBMITTER: Kasak L
PROVIDER: S-EPMC20334 | biostudies-literature | 1997 Apr
REPOSITORIES: biostudies-literature
Kasak L L Hõrak R R Kivisaar M M
Proceedings of the National Academy of Sciences of the United States of America 19970401 7
A novel experimental system to study mutation in starving bacteria was designed, relying on the activation of a promoterless phenol degradation operon of Pseudomonas putida. The Phe+ (phenol-utilizing) mutants accumulated in the starving culture of P. putida in the presence of phenol but not in the absence of it. We ruled out the possibility that the absence of phenol eliminates Phe+ mutants from the starving population. Sequence analysis of the Phe+ mutants revealed that base substitutions, del ...[more]