Ligation mediated PCR performed at low denaturation temperatures--PCR melting profiles.
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ABSTRACT:
SUBMITTER: Masny A
PROVIDER: S-EPMC203340 | biostudies-literature | 2003 Sep
REPOSITORIES: biostudies-literature
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Ligation mediated PCR performed at low denaturation temperatures--PCR melting profiles.
Masny Aleksander A Płucienniczak Andrzej A
Nucleic acids research 20030901 18
We show that using low denaturation temperatures (80-88 degrees C) during ligation mediated PCR (LM PCR) of bacterial DNA leads to the amplification of limited sets of the less stable DNA fragments. A set of electrophoretic patterns of such fragments obtained at different denaturation temperatures forms the PCR melting profile (PCR MP). A single pattern obtained for a given temperature and a set of patterns arising after application of several denaturation temperatures (PCR MP) are very specific ...[more]
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