Ontology highlight
ABSTRACT:
SUBMITTER: Notenboom V
PROVIDER: S-EPMC2034485 | biostudies-literature | 2007
REPOSITORIES: biostudies-literature
Notenboom Valerie V Hibbert Richard G RG van Rossum-Fikkert Sarah E SE Olsen Jesper V JV Mann Matthias M Sixma Titia K TK
Nucleic acids research 20070824 17
Rad18 is a ubiquitin E3 ligase that monoubiquitinates PCNA on stalled replications forks. This allows recruitment of damage-tolerant polymerases for damage bypass and DNA repair. In this activity, the Rad18 protein has to interact with Rad6, the E2 ubiquitin-conjugating enzyme, ubiquitin, PCNA and DNA. Here we analyze the biochemical interactions of specific domains of the Rad18 protein. We found that the Rad6/Rad18 complex forms stable dimers in vitro. Consistent with previous findings, both th ...[more]