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Architecture of the yeast Rrp44 exosome complex suggests routes of RNA recruitment for 3' end processing.


ABSTRACT: The eukaryotic core exosome (CE) is a conserved nine-subunit protein complex important for 3' end trimming and degradation of RNA. In yeast, the Rrp44 protein constitutively associates with the CE and provides the sole source of processive 3'-to-5' exoribonuclease activity. Here we present EM reconstructions of the core and Rrp44-bound exosome complexes. The two-lobed Rrp44 protein binds to the RNase PH domain side of the exosome and buttresses the bottom of the exosome-processing chamber. The Rrp44 C-terminal body part containing an RNase II-type active site is anchored to the exosome through a conserved set of interactions mainly to the Rrp45 and Rrp43 subunit, whereas the Rrp44 N-terminal head part is anchored to the Rrp41 subunit and may function as a roadblock to restrict access of RNA to the active site in the body region. The Rrp44-exosome (RE) architecture suggests an active site sequestration mechanism for strict control of 3' exoribonuclease activity in the RE complex.

SUBMITTER: Wang HW 

PROVIDER: S-EPMC2040474 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature

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Architecture of the yeast Rrp44 exosome complex suggests routes of RNA recruitment for 3' end processing.

Wang Hong-Wei HW   Wang Jianjun J   Ding Fang F   Callahan Kevin K   Bratkowski Matthew A MA   Butler J Scott JS   Nogales Eva E   Ke Ailong A  

Proceedings of the National Academy of Sciences of the United States of America 20071017 43


The eukaryotic core exosome (CE) is a conserved nine-subunit protein complex important for 3' end trimming and degradation of RNA. In yeast, the Rrp44 protein constitutively associates with the CE and provides the sole source of processive 3'-to-5' exoribonuclease activity. Here we present EM reconstructions of the core and Rrp44-bound exosome complexes. The two-lobed Rrp44 protein binds to the RNase PH domain side of the exosome and buttresses the bottom of the exosome-processing chamber. The R  ...[more]

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