Ontology highlight
ABSTRACT:
SUBMITTER: Wang HW
PROVIDER: S-EPMC2040474 | biostudies-literature | 2007 Oct
REPOSITORIES: biostudies-literature
Wang Hong-Wei HW Wang Jianjun J Ding Fang F Callahan Kevin K Bratkowski Matthew A MA Butler J Scott JS Nogales Eva E Ke Ailong A
Proceedings of the National Academy of Sciences of the United States of America 20071017 43
The eukaryotic core exosome (CE) is a conserved nine-subunit protein complex important for 3' end trimming and degradation of RNA. In yeast, the Rrp44 protein constitutively associates with the CE and provides the sole source of processive 3'-to-5' exoribonuclease activity. Here we present EM reconstructions of the core and Rrp44-bound exosome complexes. The two-lobed Rrp44 protein binds to the RNase PH domain side of the exosome and buttresses the bottom of the exosome-processing chamber. The R ...[more]