Collective migration of an epithelial monolayer in response to a model wound.

ABSTRACT: Using an original microfabrication-based technique, we experimentally study situations in which a virgin surface is presented to a confluent epithelium with no damage made to the cells. Although inspired by wound-healing experiments, the situation is markedly different from classical scratch wounding because it focuses on the influence of the free surface and uncouples it from the other possible contributions such as cell damage and/or permeabilization. Dealing with Madin-Darby canine kidney cells on various surfaces, we found that a sudden release of the available surface is sufficient to trigger collective motility. This migration is independent of the proliferation of the cells that mainly takes place on the fraction of the surface initially covered. We find that this motility is charac

SUBMITTER: Poujade M 

PROVIDER: S-EPMC2042149 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature