Ontology highlight
ABSTRACT:
SUBMITTER: Guglielmi B
PROVIDER: S-EPMC2042162 | biostudies-literature | 2007 Oct
REPOSITORIES: biostudies-literature
Guglielmi Benjamin B Soutourina Julie J Esnault Cyril C Werner Michel M
Proceedings of the National Academy of Sciences of the United States of America 20070927 41
The transcription initiation and elongation steps of protein-coding genes usually rely on unrelated protein complexes. However, the TFIIS elongation factor is implicated in both processes. We found that, in the absence of the Med31 Mediator subunit, yeast cells required the TFIIS polymerase II (Pol II)-binding domain but not its RNA cleavage stimulatory activity that is associated with its elongation function. We also found that the TFIIS Pol II-interacting domain was needed for the full recruit ...[more]