Ontology highlight
ABSTRACT:
SUBMITTER: Sickmier EA
PROVIDER: S-EPMC2043114 | biostudies-literature | 2006 Jul
REPOSITORIES: biostudies-literature
Sickmier E Allen EA Frato Katherine E KE Shen Haihong H Paranawithana Shanthi R SR Green Michael R MR Kielkopf Clara L CL
Molecular cell 20060701 1
The essential pre-mRNA splicing factor, U2AF(65), guides the early stages of splice site choice by recognizing a polypyrimidine (Py) tract consensus sequence near the 3' splice site. Since Py tracts are relatively poorly conserved in higher eukaryotes, U2AF(65) is faced with the problem of specifying uridine-rich sequences, yet tolerating a variety of nucleotide substitutions found in natural Py tracts. To better understand these apparently contradictory RNA binding characteristics, the X-ray st ...[more]