Bimodal regulation of RAF by CNK in Drosophila.
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ABSTRACT:
SUBMITTER: Douziech M
PROVIDER: S-EPMC204489 | biostudies-literature | 2003 Oct
REPOSITORIES: biostudies-literature
Publications
Bimodal regulation of RAF by CNK in Drosophila.
Douziech Mélanie M Roy François F Laberge Gino G Lefrançois Martin M Armengod Anne-Valérie AV Therrien Marc M
The EMBO journal 20031001 19
Connector enhancer of KSR (CNK) is a multidomain-containing protein previously identified as a positive regulator of the RAS/MAPK pathway in Drosophila. Using transfection experiments and an RNAi-based rescue assay in Drosophila S2 cells, we demonstrate that CNK has antagonistic properties with respect to RAF activity. We show that CNK's N-terminal region contains two domains (SAM and CRIC) that are essential for RAF function. Unexpectedly, we also report that the C-terminal region of CNK contai ...[more]
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