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Translational genomics to develop a Salmonella enterica serovar Paratyphi A multiplex polymerase chain reaction assay.


ABSTRACT: The use of pathogen genome sequence data for the control and management of infections remains an ongoing challenge. We describe a broadly applicable, web-enabled approach that can be used to develop bacteria-specific polymerase chain reaction (PCR) assays. Salmonella enterica Paratyphi A has emerged as a major cause of enteric fever in Asia. Culture-based diagnosis is slow and frequently negative in patients with suspected typhoid and paratyphoid fever, potentially compromising patient management and public health. We used the MobilomeFINDER web-server to perform in silico subtractive hybridization, thus identifying 43 protein-coding sequences (CDSs) that were present in two Paratyphi A strains but not in other sequenced Salmonella genomes. After exclusion of 29 CDSs found to be variably present in Paratyphi A strains by microarray hybridization and grouping of remaining CDSs by genomic location, four dispersed targets (stkF, spa2473, spa2539, hsdM) were used to develop a highly discriminatory multiplex PCR assay. All 52 Paratyphi A strains within the diverse panel investigated produced one of two pathognomonic four-band signatures. Given rapid and ongoing expansion of DNA and comparative genomics databases, our universally accessible web-server-supported do-it-yourself approach offers the potential to contribute significantly to the rapid development of species-, serovar-, or pathotype-specific PCR assays targeting pre-existing and emerging bacterial pathogens.

SUBMITTER: Ou HY 

PROVIDER: S-EPMC2049048 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature

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Translational genomics to develop a Salmonella enterica serovar Paratyphi A multiplex polymerase chain reaction assay.

Ou Hong-Yu HY   Ju Cindy Teh Shuan CT   Thong Kwai-Lin KL   Ahmad Norazah N   Deng Zixin Z   Barer Michael R MR   Rajakumar Kumar K  

The Journal of molecular diagnostics : JMD 20071101 5


The use of pathogen genome sequence data for the control and management of infections remains an ongoing challenge. We describe a broadly applicable, web-enabled approach that can be used to develop bacteria-specific polymerase chain reaction (PCR) assays. Salmonella enterica Paratyphi A has emerged as a major cause of enteric fever in Asia. Culture-based diagnosis is slow and frequently negative in patients with suspected typhoid and paratyphoid fever, potentially compromising patient managemen  ...[more]

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