Unknown

Dataset Information

0

Assembling an intermediate filament network by dynamic cotranslation.


ABSTRACT: We have been able to observe the dynamic interactions between a specific messenger RNA (mRNA) and its protein product in vivo by studying the synthesis and assembly of peripherin intermediate filaments (IFs). The results show that peripherin mRNA-containing particles (messenger ribonucleoproteins [mRNPs]) move mainly along microtubules (MT). These mRNPs are translationally silent, initiating translation when they cease moving. Many peripherin mRNPs contain multiple mRNAs, possibly amplifying the total amount of protein synthesized within these "translation factories." This mRNA clustering is dependent on MT, regulatory sequences within the RNA and the nascent protein. Peripherin is cotranslationally assembled into insoluble, nonfilamentous particles that are precursors to the long IF that form extensive cytoskeletal networks. The results show that the motility and targeting of peripherin mRNPs, their translational control, and the assembly of an IF cytoskeletal system are linked together in a process we have termed dynamic cotranslation.

SUBMITTER: Chang L 

PROVIDER: S-EPMC2063706 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Assembling an intermediate filament network by dynamic cotranslation.

Chang Lynne L   Shav-Tal Yaron Y   Trcek Tatjana T   Singer Robert H RH   Goldman Robert D RD  

The Journal of cell biology 20060201 5


We have been able to observe the dynamic interactions between a specific messenger RNA (mRNA) and its protein product in vivo by studying the synthesis and assembly of peripherin intermediate filaments (IFs). The results show that peripherin mRNA-containing particles (messenger ribonucleoproteins [mRNPs]) move mainly along microtubules (MT). These mRNPs are translationally silent, initiating translation when they cease moving. Many peripherin mRNPs contain multiple mRNAs, possibly amplifying the  ...[more]

Similar Datasets

| S-EPMC5461013 | biostudies-literature
2019-01-16 | GSE125133 | GEO
| S-EPMC2776705 | biostudies-literature
2019-01-16 | GSE125132 | GEO
2019-01-16 | GSE125131 | GEO
| S-EPMC2426950 | biostudies-literature
| S-EPMC2390850 | biostudies-literature
| S-EPMC5043008 | biostudies-other
| S-EPMC6207411 | biostudies-literature
| S-EPMC10281669 | biostudies-literature