Ontology highlight
ABSTRACT:
SUBMITTER: Rodriguez AJ
PROVIDER: S-EPMC2064499 | biostudies-literature | 2006 Oct
REPOSITORIES: biostudies-literature
Rodriguez Alexis J AJ Shenoy Shailesh M SM Singer Robert H RH Condeelis John J
The Journal of cell biology 20061001 1
The role of mRNA localization is presumably to effect cell asymmetry by synthesizing proteins in specific cellular compartments. However, protein synthesis has never been directly demonstrated at the sites of mRNA localization. To address this, we developed a live cell method for imaging translation of beta-actin mRNA. Constructs coding for beta-actin, containing tetracysteine motifs, were transfected into C2C12 cells, and sites of nascent polypeptide chains were detected using the biarsenial dy ...[more]