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Visualization of PS/?-Secretase Activity in Living Cells.

ABSTRACT: A change in Presenilin (PS)/?-secretase activity is linked to essential biological events as well as to the progression of many diseases. However, not much is known about how PS/?-secretase activity is spatiotemporally regulated in cells. One of the limitations is lack of tools to directly monitor dynamic behavior of the PS/?-secretase in intact/live cells. Here we present successful development and validation of the Förster resonance energy transfer (FRET)-based biosensors that enable quantitative monitoring of endogenous PS/?-secretase activity in live cells longitudinally on a cell-by-cell basis. Using these FRET biosensors, we uncovered that PS/?-secretase activity is heterogeneously regulated among live neurons.

SUBMITTER: Maesako M

PROVIDER: S-EPMC7235286 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Visualization of PS/γ-Secretase Activity in Living Cells.

Maesako Masato M Sekula Nicole M NM Aristarkhova Anna A Feschenko Polina P Anderson Lauren C LC Berezovska Oksana O

iScience 20200507 6

A change in Presenilin (PS)/γ-secretase activity is linked to essential biological events as well as to the progression of many diseases. However, not much is known about how PS/γ-secretase activity is spatiotemporally regulated in cells. One of the limitations is lack of tools to directly monitor dynamic behavior of the PS/γ-secretase in intact/live cells. Here we present successful development and validation of the Förster resonance energy transfer (FRET)-based biosensors that enable quantitat ...[more]

PMID: 32438286

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