Rapid T cell receptor-mediated SHP-1 S591 phosphorylation regulates SHP-1 cellular localization and phosphatase activity.
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ABSTRACT:
SUBMITTER: Liu Y
PROVIDER: S-EPMC2084461 | biostudies-literature | 2007 Sep
REPOSITORIES: biostudies-literature
Publications
Rapid T cell receptor-mediated SHP-1 S591 phosphorylation regulates SHP-1 cellular localization and phosphatase activity.
Liu Yin Y Kruhlak Michael J MJ Hao Jian-Jiang JJ Shaw Stephen S
Journal of leukocyte biology 20070615 3
Since the tyrosine phosphatase SHP-1 plays a major role in regulating T cell signaling, we investigated regulation thereof by Ser/Thr phosphorylation. We found that T cell receptor (TCR) stimulation induced fast (<or=1 min) and transient phosphorylation of SHP-1 S591 in both Jurkat and human peripheral blood T-cells (PBT). Phosphorylation of S591 in T-cells could be mediated artificially by a constitutive active PKC-theta construct, but the dose dependence of inhibition by PKC inhibitors indicat ...[more]
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