Ontology highlight
ABSTRACT:
SUBMITTER: Mei R
PROVIDER: S-EPMC208741 | biostudies-literature | 2003 Sep
REPOSITORIES: biostudies-literature
Mei Rui R Hubbell Earl E Bekiranov Stefan S Mittmann Mike M Christians Fred C FC Shen Mei-Mei MM Lu Gang G Fang Joy J Liu Wei-Min WM Ryder Tom T Kaplan Paul P Kulp David D Webster Teresa A TA
Proceedings of the National Academy of Sciences of the United States of America 20030919 20
High-density oligonucleotide microarrays enable simultaneous monitoring of expression levels of tens of thousands of transcripts. For accurate detection and quantitation of transcripts in the presence of cellular mRNA, it is essential to design microarrays whose oligonucleotide probes produce hybridization intensities that accurately reflect the concentration of original mRNA. We present a model-based approach that predicts optimal probes by using sequence and empirical information. We construct ...[more]