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Single metallic nanoparticle imaging for protein detection in cells.

ABSTRACT: We performed a visualization of membrane proteins labeled with 10-nm gold nanoparticles in cells, using an all-optical method based on photothermal interference contrast. The high sensitivity of the method and the stability of the signals allows 3D imaging of individual nanoparticles without the drawbacks of photobleaching and blinking inherent to fluorescent markers. A simple analytical model is derived to account for the measurements of the signal amplitude and the spatial resolution. The photothermal interference contrast method provides an efficient, reproducible, and promising way to visualize low amounts of proteins in cells by optical means.

SUBMITTER: Cognet L

PROVIDER: S-EPMC208760 | biostudies-literature | 2003 Sep

REPOSITORIES: biostudies-literature

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Single metallic nanoparticle imaging for protein detection in cells.

Cognet L L Tardin C C Boyer D D Choquet D D Tamarat P P Lounis B B

Proceedings of the National Academy of Sciences of the United States of America 20030917 20

We performed a visualization of membrane proteins labeled with 10-nm gold nanoparticles in cells, using an all-optical method based on photothermal interference contrast. The high sensitivity of the method and the stability of the signals allows 3D imaging of individual nanoparticles without the drawbacks of photobleaching and blinking inherent to fluorescent markers. A simple analytical model is derived to account for the measurements of the signal amplitude and the spatial resolution. The phot ...[more]

PMID: 13679586

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