Dataset Information

In vitro microscopic imaging of enhanced thrombolysis with 120-kHz ultrasound in a human clot model.

ABSTRACT: Substantial enhancement of recombinant tissue plasminogen activator (rt-PA) thrombolysis can be achieved with ultrasound, suggesting its use as an adjunctive treatment in thrombolytic therapy for stroke. A microscopic visualization method was used to measure the lysis of human whole-blood clots treated with human fresh frozen plasma (HFFP), rt-PA, and 120-kHz ultrasound for 30 min at T = 37 ° C. The clot-plasma interface was imaged using an inverted optical microscope and the thrombolytic front analyzed as a function of time. Ultrasound treatment significantly enhanced the mean lytic rate from 0.5 to 3.4 ?m/min (a 580% change) compared with rt-PA treatment alone.

SUBMITTER: Cheng JY

PROVIDER: S-EPMC2137158 | biostudies-literature | 2005 Jan

REPOSITORIES: biostudies-literature

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In vitro microscopic imaging of enhanced thrombolysis with 120-kHz ultrasound in a human clot model.

Cheng Jason Y JY Shaw George J GJ Holland Christy K CK

Acoustics research letters online : ARLO 20050101 1

Substantial enhancement of recombinant tissue plasminogen activator (rt-PA) thrombolysis can be achieved with ultrasound, suggesting its use as an adjunctive treatment in thrombolytic therapy for stroke. A microscopic visualization method was used to measure the lysis of human whole-blood clots treated with human fresh frozen plasma (HFFP), rt-PA, and 120-kHz ultrasound for 30 min at T = 37 ° C. The clot-plasma interface was imaged using an inverted optical microscope and the thrombolytic front ...[more]

PMID: 18080003

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Project description:PurposeContrast-enhanced ultrasound imaging has expanded the diagnostic potential of ultrasound by enabling real-time imaging and quantification of tissue perfusion. Several perfusion models and curve fitting methods have been developed to quantify the temporal behavior of tracer signal and standardize perfusion quantification. While the least-squares approach has traditionally been applied for curve fitting, it can be inadequate for noisy and complex data. Moreover, previous research suggests that certain perfusion models may be more relevant depending on the organ or tissue imaged. We propose a multi-model framework to select the most appropriate perfusion model and curve fitting method for each diagnostic application.MethodsOur multi-model approach uses a system identification method, which estimates perfusion parameters from the model with the best fit to a given time-intensity curve. We compared current perfusion quantification methods that use a single perfusion model and curve fitting method and our proposed multi-model framework on bolus 3D dynamic contrast-enhanced ultrasound (DCE-US) in vivo images obtained in mice implanted with a colon cancer, as well as on simulation data. The quality of fit in estimating perfusion parameters was evaluated using the Spearman correlation coefficient, the coefficient of determination (R2 ), and the normalized root-mean-square error (NRMSE) to ensure that the multi-model framework finds the best perfusion model and curve fitting algorithm.ResultsOur multi-model framework outperforms conventional single perfusion model approaches with least-squares optimization, providing more robust perfusion parameter estimation. R2 and NRMSE are 0.98 and 0.18, respectively, for our proposed method. By comparison, the performance of the traditional approach is much more dependent upon the selection of the appropriate model. The R2 and NRMSE are 0.91 and 0.31, respectively.ConclusionsThe proposed multi-model framework for perfusion modeling outperforms the current approach of single perfusion modeling using least-squares optimization and more robustly estimates perfusion parameters when using empiric data labeled by an expert as the gold standard. Our technique is minimally sensitive to issues affecting the accuracy of perfusion parameter estimation, including rise time, noise, region of interest size, and frame rate. This framework could be of key utility in modeling different perfusion systems in different tissues and organs.

Dataset Information

In vitro microscopic imaging of enhanced thrombolysis with 120-kHz ultrasound in a human clot model.

Publications

In vitro microscopic imaging of enhanced thrombolysis with 120-kHz ultrasound in a human clot model.

Similar Datasets

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