Dataset Information

Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines.

ABSTRACT:

Background

Fruit coloration of red-skinned grapevines is mainly due to anthocyanin pigments. We analysed a panel of nine cultivars that included extreme phenotypes for berry colour, ranging from green (absence of anthocyanins) to red, purple, violet and blue. Expression of six genes of the anthocyanin pathway coding for flavanone-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H), flavonoid 3',5'-hydroxylase (F3'5'H), UDP-glucose:flavonoid-3-O-glucosyltransferase (UFGT), glutathione-S-transferase (GST), O-methyltransferase (OMT) and four transcription factors (MybA, MybB, MybC, MybD) was analysed by quantitative RT-PCR at four developmental stages from before the onset of ripening until full maturity and compared to anthocyanin metabolites.

Results

Total anthocyanin content at full maturity correlated well with the cumulative expression of F3H, UFGT and GST throughout ripening. Transcripts of the last two genes were absent in the green-skinned cultivar 'Sauvignonasse', also known as 'Tocai friulano', and were at least 10-fold less abundant in pale red cultivars, such as 'Pinot gris' and 'Gewürztraminer', compared to fully coloured cultivars. Predominance of tri-hydroxylated anthocyanins (delphinidin, petunidin and malvidin) in cultivars bearing dark berries with violet and blue hue was associated with higher ratios of F3'5'H/F3'H transcription, compared to red-skinned cultivars. Higher levels of OMT transcripts were observed in berries of cultivars that accumulated methoxylated forms of anthocyanins more abundantly than non-methoxylated forms.

Conclusion

Colour variation of the grape berry conforms to a peculiar pattern of genotype-specific expression of the whole set of anthocyanin genes in a direct transcript-metabolite-phenotype relationship. Cumulative mRNA levels of the structural genes and their relative abundance throughout ripening explained per se the final phenotype for anthocyanin content, anthocyanin composition, colour intensity and colour hue of grapes at berry maturity.

SUBMITTER: Castellarin SD

PROVIDER: S-EPMC2147006 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature

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Publications

Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines.

Castellarin Simone D SD Di Gaspero Gabriele G

BMC plant biology 20070830

<h4>Background</h4>Fruit coloration of red-skinned grapevines is mainly due to anthocyanin pigments. We analysed a panel of nine cultivars that included extreme phenotypes for berry colour, ranging from green (absence of anthocyanins) to red, purple, violet and blue. Expression of six genes of the anthocyanin pathway coding for flavanone-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H), flavonoid 3',5'-hydroxylase (F3'5'H), UDP-glucose:flavonoid-3-O-glucosyltransferase (UFGT), glutathione-S-tr ...[more]

PMID: 17760970

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Project description:BACKGROUND:The cultivated potato Solanum tuberosum L. is the fourth most important crop worldwide. Anthocyanins synthesis and accumulation in potato tissues are considered as one of important traits related to stress resistance and nutritional value. It is considered that the major regulatory gene for anthocyanin biosynthesis is R2R3 MYB-encoding gene StAN1. However, the genetic control of pigmentation of different potato tissues is substantially under investigated. The development of genetic markers for breeding of potato with specific pigmentation pattern remains an actual task. RESULTS:We investigated 36 potato varieties and hybrids with different pigmentation of tubers and leaves. Sequence organization of regulatory R2R3 MYB (StAN1, StMYBA1, StMYB113), bHLH (StbHLH1, StJAF13) and WD40 (StWD40) genes potentially controlling anthocyanin biosynthesis has been evaluated. The results demonstrated a high variability in the StAN1 third exon and promoter region with the exception for 35?bp, containing elements for the transcription start and activation of gene expression in roots. The analysis of transcriptional activity of genes coding R2R3 MYBs, bHLHs and WD40 transcriptional factors in leaves of eight potato genotypes with different anthocyanin pigmentation was performed. The results showed a relation between the gene expression level and plant pigmentation only for the StAN1 and StWD40 genes, while other studied genes had either strong expression in all varieties and hybrids (StMYBA1, StbHLH1 and StJAF13) or they were not expressed at all (StMYB113). CONCLUSIONS:It was found that StAN1 is the major regulatory gene controlling potato anthocyanin synthesis. However, diagnostic markers developed for the functional StAN1 alleles (StAN1777 and StAN1816) can not be used efficiently for prediction of potato pigmentation patterns. It is likely that the sequence organization of StAN1 promoter is important for anthocyanin synthesis control and the development of additional diagnostic markers is necessary.

Project description:Monastrell grapevines grafted on the rootstocks 140Ru, 1103P, 41B, 110R, and 161-49C were subjected to regulated deficit irrigation (RDI) and partial root-zone irrigation (PRI). We analyzed the effects of the rootstock and irrigation method on the phenolic concentration in different berry tissues, its dilution/concentration due to the berry size, the anatomical and morphological traits of berries related to the phenolic compounds concentration, and the relationships of all these parameters with the final berry and wine phenolic content. The rootstock had an important effect on the accumulation of total phenolic compounds and anthocyanins in the skin (berries from 110R and 140Ru had the highest values). Moreover, the rootstock modified some anatomical and morphological characteristics that had a direct relationship with the final phenolic compounds concentration in the must. Large grapes and high must percentages (110R and 140Ru) produced a dilution effect, whereas small berries and a low must percentage increased the concentration (161-49C). For 110R, the small size of the cells of the epidermis and hypodermis in the grapes also could have contributed to the high phenolic compounds concentration in the skin. The percentage of cells in the skin with a uniform coloration was positively correlated with its total phenolic compounds and anthocyanins concentration and also with the phenolic quality of the wine. The PRI modified some specific morphological/anatomical skin/berry traits, and these may have contributed to important changes in the final concentration of phenolic compounds, depending on the rootstock. The better phenolic quality of the must and wines observed in some rootstocks under PRI could be due to smaller cells in the epidermis and hypodermis of the skin (161-49C), a higher percentage of cells with a uniform coloration in the hypodermis (110R), or a lower number of seeds per berry (161-49C). In contrast, the lower phenolic compounds concentration in the must of grapes observed in the most vigorous rootstocks under PRI could be due to a greater thickness of the epidermis (140Ru), greater cuticle thickness (41B), a higher number of seeds (140Ru), a lower skin/pulp ratio and percentage of skin (140Ru), a greater percentage of cells in the epidermis without coloration or with large inclusions, and a lower percentage of cells with a uniform coloration in the epidermis (140Ru). The final quality of the grape is related to some changes in histological and morphological aspects of the grape produced by the rootstock and irrigation strategy.

Dataset Information

Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines.

Background

Results

Conclusion

Publications

Transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines.

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