Ontology highlight
ABSTRACT:
SUBMITTER: Hobman JL
PROVIDER: S-EPMC2168620 | biostudies-literature | 2007 Dec
REPOSITORIES: biostudies-literature
Hobman Jon L JL Patel Mala D MD Hidalgo-Arroyo G Aida GA Cariss S James L SJ Avison Matthew B MB Penn Charles W CW Constantinidou Chrystala C
Journal of bacteriology 20071005 24
The use of whole-genome microarrays for monitoring mutagenized or otherwise engineered genetic derivatives is a potentially powerful tool for checking genomic integrity. Using comparative genomic hybridization of a number of unrelated, directed deletion mutants in Escherichia coli K-12 MG1655, we identified unintended secondary genomic deletions in the flhDC region in delta fnr, delta crp, and delta creB mutants. These deletions were confirmed by PCR and phenotypic tests. Our findings show that ...[more]