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MCAK associates with the tips of polymerizing microtubules.

ABSTRACT: MCAK is a member of the kinesin-13 family of microtubule (MT)-depolymerizing kinesins. We show that the potent MT depolymerizer MCAK tracks (treadmills) with the tips of polymerizing MTs in living cells. Tip tracking of MCAK is inhibited by phosphorylation and is dependent on the extreme COOH-terminal tail of MCAK. Tip tracking is not essential for MCAK's MT-depolymerizing activity. We propose that tip tracking is a mechanism by which MCAK is preferentially localized to regions of the cell that modulate the plus ends of MTs.

SUBMITTER: Moore AT

PROVIDER: S-EPMC2171944 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature

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MCAK associates with the tips of polymerizing microtubules.

Moore Ayana T AT Rankin Kathleen E KE von Dassow George G Peris Leticia L Wagenbach Michael M Ovechkina Yulia Y Andrieux Annie A Job Didier D Wordeman Linda L

The Journal of cell biology 20050501 3

MCAK is a member of the kinesin-13 family of microtubule (MT)-depolymerizing kinesins. We show that the potent MT depolymerizer MCAK tracks (treadmills) with the tips of polymerizing MTs in living cells. Tip tracking of MCAK is inhibited by phosphorylation and is dependent on the extreme COOH-terminal tail of MCAK. Tip tracking is not essential for MCAK's MT-depolymerizing activity. We propose that tip tracking is a mechanism by which MCAK is preferentially localized to regions of the cell that ...[more]

PMID: 15883193

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