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Functional role of epsilon-tubulin in the assembly of the centriolar microtubule scaffold.

ABSTRACT: Centrioles and basal bodies fascinate by their spectacular architecture, featuring an arrangement of nine microtubule triplets into an axial symmetry, whose biogenesis relies on yet elusive mechanisms. However, the recent discovery of new tubulins, such as delta-, epsilon-, or eta-tubulin, could constitute a breakthrough for deciphering the assembly steps of this unconventional microtubule scaffold. Here, we report the functional analysis in vivo of epsilon-tubulin, based on gene silencing in Paramecium, which demonstrates that this protein, which localizes at the basal bodies, is essential for the assembly and anchorage of the centriolar microtubules.

SUBMITTER: Dupuis-Williams P

PROVIDER: S-EPMC2173240 | biostudies-literature | 2002 Sep

REPOSITORIES: biostudies-literature

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Functional role of epsilon-tubulin in the assembly of the centriolar microtubule scaffold.

Dupuis-Williams Pascale P Fleury-Aubusson Anne A de Loubresse Nicole Garreau NG Geoffroy Hélène H Vayssié Laurence L Galvani Angélique A Espigat Aude A Rossier Jean J

The Journal of cell biology 20020901 7

Centrioles and basal bodies fascinate by their spectacular architecture, featuring an arrangement of nine microtubule triplets into an axial symmetry, whose biogenesis relies on yet elusive mechanisms. However, the recent discovery of new tubulins, such as delta-, epsilon-, or eta-tubulin, could constitute a breakthrough for deciphering the assembly steps of this unconventional microtubule scaffold. Here, we report the functional analysis in vivo of epsilon-tubulin, based on gene silencing in Pa ...[more]

PMID: 12356863

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