Unknown

Dataset Information

0

Role of CAP350 in centriolar tubule stability and centriole assembly.


ABSTRACT: BACKGROUND: Centrioles are microtubule-based cylindrical structures composed of nine triplet tubules and are required for the formation of the centrosome, flagella and cilia. Despite theirs importance, centriole biogenesis is poorly understood. Centrosome duplication is initiated at the G1/S transition by the sequential recruitment of a set of conserved proteins under the control of the kinase Plk4. Subsequently, the procentriole is assembled by the polymerization of centriolar tubules via an unknown mechanism involving several tubulin paralogs. METHODOLOGY/PRINCIPAL FINDINGS: Here, we developed a cellular assay to study centrosome duplication and procentriole stability based on its sensitivity to the microtubule-depolymerizing drug nocodazole. By using RNA interference experiments, we show that the stability of growing procentrioles is regulated by the microtubule-stabilizing protein CAP350, independently of hSAS-6 and CPAP which initiate procentriole growth. Furthermore, our analysis reveals the critical role of centriolar tubule stability for an efficient procentriole growth. CONCLUSIONS/SIGNIFICANCE: CAP350 belongs to a new class of proteins which associate and stabilize centriolar tubules to control centriole duplication.

SUBMITTER: Le Clech M 

PROVIDER: S-EPMC2586089 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature

altmetric image

Publications

Role of CAP350 in centriolar tubule stability and centriole assembly.

Le Clech Mikael M  

PloS one 20081204 12


<h4>Background</h4>Centrioles are microtubule-based cylindrical structures composed of nine triplet tubules and are required for the formation of the centrosome, flagella and cilia. Despite theirs importance, centriole biogenesis is poorly understood. Centrosome duplication is initiated at the G1/S transition by the sequential recruitment of a set of conserved proteins under the control of the kinase Plk4. Subsequently, the procentriole is assembled by the polymerization of centriolar tubules vi  ...[more]

Similar Datasets

| S-EPMC4472012 | biostudies-literature
| S-EPMC2682601 | biostudies-literature
| S-EPMC7135989 | biostudies-literature
| S-EPMC7500955 | biostudies-literature
| S-EPMC4574112 | biostudies-literature
| S-EPMC2173240 | biostudies-literature
| S-SCDT-10_15252-EMBR_202256317 | biostudies-other
2023-05-10 | PXD031920 | Pride
2012-03-01 | GSE32452 | GEO
2012-03-01 | E-GEOD-32452 | biostudies-arrayexpress