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Secretory vesicle transport velocity in living cells depends on the myosin-V lever arm length.


ABSTRACT: Myosins are molecular motors that exert force against actin filaments. One widely conserved myosin class, the myosin-Vs, recruits organelles to polarized sites in animal and fungal cells. However, it has been unclear whether myosin-Vs actively transport organelles, and whether the recently challenged lever arm model developed for muscle myosin applies to myosin-Vs. Here we demonstrate in living, intact yeast that secretory vesicles move rapidly toward their site of exocytosis. The maximal speed varies linearly over a wide range of lever arm lengths genetically engineered into the myosin-V heavy chain encoded by the MYO2 gene. Thus, secretory vesicle polarization is achieved through active transport by a myosin-V, and the motor mechanism is consistent with the lever arm model.

SUBMITTER: Schott DH 

PROVIDER: S-EPMC2173574 | biostudies-literature | 2002 Jan

REPOSITORIES: biostudies-literature

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Secretory vesicle transport velocity in living cells depends on the myosin-V lever arm length.

Schott Daniel H DH   Collins Ruth N RN   Bretscher Anthony A  

The Journal of cell biology 20020107 1


Myosins are molecular motors that exert force against actin filaments. One widely conserved myosin class, the myosin-Vs, recruits organelles to polarized sites in animal and fungal cells. However, it has been unclear whether myosin-Vs actively transport organelles, and whether the recently challenged lever arm model developed for muscle myosin applies to myosin-Vs. Here we demonstrate in living, intact yeast that secretory vesicles move rapidly toward their site of exocytosis. The maximal speed  ...[more]

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