Secretory vesicle transport velocity in living cells depends on the myosin-V lever arm length.
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ABSTRACT:
SUBMITTER: Schott DH
PROVIDER: S-EPMC2173574 | biostudies-literature | 2002 Jan
REPOSITORIES: biostudies-literature
Publications
Secretory vesicle transport velocity in living cells depends on the myosin-V lever arm length.
Schott Daniel H DH Collins Ruth N RN Bretscher Anthony A
The Journal of cell biology 20020107 1
Myosins are molecular motors that exert force against actin filaments. One widely conserved myosin class, the myosin-Vs, recruits organelles to polarized sites in animal and fungal cells. However, it has been unclear whether myosin-Vs actively transport organelles, and whether the recently challenged lever arm model developed for muscle myosin applies to myosin-Vs. Here we demonstrate in living, intact yeast that secretory vesicles move rapidly toward their site of exocytosis. The maximal speed ...[more]
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