Ontology highlight
ABSTRACT:
SUBMITTER: Yu D
PROVIDER: S-EPMC218769 | biostudies-literature | 2003 Oct
REPOSITORIES: biostudies-literature
Proceedings of the National Academy of Sciences of the United States of America 20030930 21
Human cytomegalovirus has a complex double-stranded DNA genome of approximately 240,000 bp that contains approximately 150 ORFs likely to encode proteins, most of whose functions are not well understood. We have used an infectious bacterial artificial chromosome to introduce 413 defined insertion and substitution mutations into the human cytomegalovirus AD169 genome by random and site-directed transposon mutagenesis. Mutations were produced in all unique ORFs with a high probability of encoding ...[more]