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DNA-dependent protein kinase activity is not required for immunoglobulin class switching.


ABSTRACT: Class switch recombination (CSR), similar to V(D)J recombination, is thought to involve DNA double strand breaks and repair by the nonhomologous end-joining pathway. A key component of this pathway is DNA-dependent protein kinase (DNA-PK), consisting of a catalytic subunit (DNA-PKcs) and a DNA-binding heterodimer (Ku70/80). To test whether DNA-PKcs activity is essential for CSR, we examined whether IgM(+) B cells from scid mice with site-directed H and L chain transgenes were able to undergo CSR. Although B cells from these mice were shown to lack DNA-PKcs activity, they were able to switch from IgM to IgG or IgA with close to the same efficiency as B cells from control transgenic and nontransgenic scid/+ mice, heterozygous for the scid mutation. We conclude that CSR, unlike V(D)J recombination, can readily occur in the absence of DNA-PKcs activity. We suggest nonhomologous end joining may not be the (primary or only) mechanism used to repair DNA breaks during CSR.

SUBMITTER: Bosma GC 

PROVIDER: S-EPMC2194268 | biostudies-literature | 2002 Dec

REPOSITORIES: biostudies-literature

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DNA-dependent protein kinase activity is not required for immunoglobulin class switching.

Bosma Gayle C GC   Kim Jiyoon J   Urich Teresa T   Fath Donna M DM   Cotticelli Maria G MG   Ruetsch Norman R NR   Radic Marko Z MZ   Bosma Melvin J MJ  

The Journal of experimental medicine 20021201 11


Class switch recombination (CSR), similar to V(D)J recombination, is thought to involve DNA double strand breaks and repair by the nonhomologous end-joining pathway. A key component of this pathway is DNA-dependent protein kinase (DNA-PK), consisting of a catalytic subunit (DNA-PKcs) and a DNA-binding heterodimer (Ku70/80). To test whether DNA-PKcs activity is essential for CSR, we examined whether IgM(+) B cells from scid mice with site-directed H and L chain transgenes were able to undergo CSR  ...[more]

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