Ontology highlight
ABSTRACT:
SUBMITTER: Ziegelin G
PROVIDER: S-EPMC219471 | biostudies-literature | 2003 Oct
REPOSITORIES: biostudies-literature
Ziegelin Günter G Niedenzu Timo T Lurz Rudi R Saenger Wolfram W Lanka Erich E
Nucleic acids research 20031001 20
In the known monoclinic crystals the 3-dimensional structure of the hexameric, replicative helicase RepA encoded by plasmid RSF1010 shows 6-fold rotational symmetry. In contrast, in the cubic crystal form at 2.55 A resolution described here RepA has 3-fold symmetry and consists of a trimer of dimers. To study structure-function relationships, a series of repA deletion mutants and mutations yielding single amino acid exchanges were constructed and the respective gene products were analyzed in viv ...[more]