Ontology highlight
ABSTRACT:
SUBMITTER: Sales M
PROVIDER: S-EPMC2204136 | biostudies-literature | 2007 Oct
REPOSITORIES: biostudies-literature
Sales Mark M Plecs Joseph J JJ Holton James M JM Alber Tom T
Protein science : a publication of the Protein Society 20070831 10
The previous design of an unprecedented family of two-, three-, and four-helical, right-handed coiled coils utilized nonbiological amino acids to efficiently pack spaces in the oligomer cores. Here we show that a stable, right-handed parallel tetrameric coiled coil, called RH4B, can be designed entirely using biological amino acids. The X-ray crystal structure of RH4B was determined to 1.1 Angstrom resolution using a designed metal binding site to coordinate a single Yb(2+) ion per 33-amino acid ...[more]