Ontology highlight
ABSTRACT:
SUBMITTER: Guo Z
PROVIDER: S-EPMC2206656 | biostudies-literature | 2007 Jun
REPOSITORIES: biostudies-literature
Protein science : a publication of the Protein Society 20070501 6
A nitroxide side chain (R1) has been substituted at single sites along a helix-turn-helix motif in T4 lysozyme (residues 114-135). Together with previously published data, the new sites reported complete a continuous scan through the motif. Mutants with R1 at sites 115 and 118 were selected for crystallographic analysis to identify the structural origins of the corresponding two-component EPR spectra. At 115, R1 is shown to occupy two rotamers in the room temperature crystal structure, one of wh ...[more]